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Code Name Applications Availability Size Tested species Price Grade  
EB12538 Goat Anti-AMACR Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB12538 1
EB12538 (0.5µg/ml) staining of CaCo-2 (A) and LNCaP (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12538 2
EB12538 (0.5µg/ml) staining of Human Kidney (A) and Liver (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Target Protein

Principal Names: AMACR, alpha-methylacyl-CoA racemase, AMACRD, CBAS4, RACE, RM, 2-methylacyl-CoA racemase
Official Symbol: AMACR
Accession Number(s): NP_055139.4; NP_001161067.1
Human GeneID(s): 23600

Immunogen

Peptide with sequence C-QLNSDKIIESN, from the C Terminus of the protein sequence according to NP_055139.4; NP_001161067.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: pprox 40-42kDa band observed in Human Kidney and Liver lysates, and in lysates of cell line CaCo-2 and approx 40-45kDa band observed in lysates of cell line LNCaP (calculated MW of 42.4kDa according to NP_055139.4) and 43.9kDa according to NP_001161067.1).. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human