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Goat Anti-Amyloid beta peptide (mouse) Antibody (EB11159)

Related Products: EBP11159 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11159 Goat Anti-Amyloid beta peptide (mouse) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11159 1
EB11159 (0.03µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11159 2
EB11159 (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: Abeta, Abpp, Adap, Ag, alzheimer disease amyloid A4 protein homolog, amyloid beta (A4) precursor protein, amyloid beta A4 protein, amyloidogenic glycoprotein, appican, betaApp, Cvap, E030013M08Rik, protease nexin II, App
Official Symbol: App
Accession Number(s): NP_001185752.1; NP_031497.2; NP_001185753.1; NP_001185754.1; NP_001185755.1
Non-Human GeneID(s): 11820 (mouse), 54226 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms of mAPP (NP_001185752.1; NP_031497.2; NP_001185753.1; NP_001185754.1; NP_001185755.1) and to recognize all lengths of the putative mouse Amyloid beta peptide.

Immunogen

Peptide with sequence DAEFGHDSGFEVRHQK-C, from the N Terminus of the protein sequence according to NP_001185752.1; NP_031497.2; NP_001185753.1; NP_001185754.1; NP_001185755.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 100kDa band observed in Mouse Brain lysates (calculated MW of 86.7kDa according to NP_001185752.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Mouse, Rat

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