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Code Name Applications Availability Size Tested species Price Grade  
EB07300 Goat Anti-ANILLIN / Scraps (internal) Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07300 1
EB07300 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07300 2
EB07300 Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ANILLIN, Scraps, ANLN, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, anillin (Drosophila Scraps homolog), actin binding protein
Official Symbol: ANLN
Accession Number(s): NP_061155.2
Human GeneID(s): 54443
Non-Human GeneID(s): 68743 (mouse), 307056 (rat)

Immunogen

Peptide with sequence C-TYPDDEKRKNP, from the internal region of the protein sequence according to NP_061155.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.

Immunofluorescence: Strong expression of the protein seen in the nuclei of NIH3T3 and Neuro-2a cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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