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Goat Anti-ANKK1 Antibody (EB08835)

Related Products: EBP08835 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08835 Goat Anti-ANKK1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08835 1
EB08835 (0.1µg/ml) staining of Human Substantia Nigra lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08835 2
EB08835 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ANKK1, ankyrin repeat and kinase domain containing 1, PKK2, X-kinase, protein kinase PKK2
Official Symbol: ANKK1
Accession Number(s): NP_848605.1
Human GeneID(s): 255239

Immunogen

Peptide with sequence C-SRQADPNLHEAEGKT, from the internal region of the protein sequence according to NP_848605.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 85kDa band observed in Human Brain (Substantia nigra and Amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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