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Goat Anti-APOB Antibody (EB08792)

Related Products: EBP08792 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08792 Goat Anti-APOB Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08792 1
EB08792 (0.5µg/ml) staining of Human Serum lysates. Data kindly provided by Yan Xie and NO Davidson, Washington USA. This antibody showed background below 150kDa in serum samples.
EB08792 2
EB08792 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08792 3
EB08792 (2µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: APOB, apolipoprotein B (including Ag(x) antigen), FLDB, apoB-100, apolipoprotein B
Official Symbol: APOB
Accession Number(s): NP_000375.3
Human GeneID(s): 338
Important Comments: This antibody only recognises ApoB-100 and not the cleaved chain ApoB-48.

Immunogen

Peptide with sequence C-TDLHLRYQKDKK, from the internal region of the protein sequence according to NP_000375.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Customer data showed a band corresponding to the exopected size of ApoB-100 in Human serum samples from five different individuals (calculated Mwt of 515kDa according to NP_000375.3). The smalller bands shown are believed to be degradation products of ApoB-100 (not ApoB-48). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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