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Goat Anti-APOB Antibody (EB08792)

Related Products: EBP08792 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08792 Goat Anti-APOB Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08792 1
EB08792 (0.5µg/ml) staining of Human Serum lysates. Data kindly provided by Yan Xie and NO Davidson, Washington USA. This antibody showed background below 150kDa in serum samples.
EB08792 2
EB08792 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08792 3
EB08792 (2µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: APOB, apolipoprotein B (including Ag(x) antigen), FLDB, apoB-100, apolipoprotein B
Official Symbol: APOB
Accession Number(s): NP_000375.3
Human GeneID(s): 338
Important Comments: This antibody only recognises ApoB-100 and not the cleaved chain ApoB-48.

Immunogen

Peptide with sequence C-TDLHLRYQKDKK, from the internal region of the protein sequence according to NP_000375.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Customer data showed a band corresponding to the exopected size of ApoB-100 in Human serum samples from five different individuals (calculated Mwt of 515kDa according to NP_000375.3). The smalller bands shown are believed to be degradation products of ApoB-100 (not ApoB-48). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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