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Goat Anti-APOBEC2 (aa12 - 26) Antibody (EB08357)

Related Products: EBP08357 - Immunizing Peptide, EBP08444 - Immunizing Peptide, Goat Anti-APOBEC2 (aa180 - 190) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08357 Goat Anti-APOBEC2 (aa12 - 26) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08357 1
EB08357 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: APOBEC2, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2, ARCD1, ARP1, OTTHUMP00000039773, apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2
Official Symbol: APOBEC2
Accession Number(s): NP_006780.1
Human GeneID(s): 10930
Non-Human GeneID(s): 11811 (mouse), 301226 (rat)

Immunogen

Peptide with sequence C-EAASQNGEDLENLDD, from the internal region (near the N Terminus) of the protein sequence according to NP_006780.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 26kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 25.7kDa according to NP_006780.1). Recommended concentration: 1-3µg/ml. An additional band of 90kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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FAQs

Q: Is there any cross-reactivity with other APOBEC proteins?

A: No, a BLAST search with the immunizing peptide reveals no match with any other APOBEC protein.