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Goat Anti-Arachidonate 5-lipoxygenase Antibody (EB07389)

Related Products: EBP07389 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07389 Goat Anti-Arachidonate 5-lipoxygenase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07389 1
EB07389 (0.3µg/ml) staining of Rat Stomach lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: arachidonate 5-lipoxygenase, RP11-67C2.3, ALOX5, ALOX5, 5-LO, 5LPG, LOG5, arachidonic acid 5-lipoxygenase, leukotriene A4 synthase
Official Symbol: ALOX5
Accession Number(s): NP_000689.1
Human GeneID(s): 240
Non-Human GeneID(s): 11689 (mouse), 25290 (rat)

Immunogen

Peptide with sequence C-ERNKKKQLPYYYLSPD, from the C Terminus of the protein sequence according to NP_000689.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 85kDa band observed in Human Bone Marrow and Rat Stomach lysates (calculated MW of 77.7.0kDa according to rat NP_036954). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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