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Code Name Applications Availability Size Tested species Price Grade  
EB06795 Goat Anti-Arginase I Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human $423.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06795 1

EB06795 optimised QC. Primary incubation 1 hour at room temperature.

Image A: Human Liver lysate at primary Ab concentration 0.5ugml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB06795 2
EB06795 Immunofluorescence analysis of paraformaldehyde fixed K562. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARG1, arginase, liver, HGNC:663, A-I, arginase, type I, arginase 1, liver-type arginase, type I arginase
Official Symbol: ARG1
Accession Number(s): NP_000036.2; NP_001231367.1; NP_001355949.1
Human GeneID(s): 383
Non-Human GeneID(s): 11846 (mouse), 29221 (rat)
Important Comments: Please note that ARG1 is not expressed in Human M2 macrophages, but it is in other species (eg rodents).

Immunogen

Peptide with sequence CFGLAREGNHKPID, from the C Terminus of the protein sequence according to NP_000036.2; NP_001231367.1; NP_001355949.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx.37kDa band observed in Human Liver lysates (calculated MW of 34.7kDa according to NP_000036.2) . Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of K562 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow, Pig

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