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Goat Anti-ARHU / WRCH1 Antibody (EB05375)

Related Products: EBP05375 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05375 Goat Anti-ARHU / WRCH1 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: RHOU, ras homolog gene family, member U, ARHU, WRCH1, hG28K, WRCH-1, CDC42L1, FLJ10616, DJ646B12.2, fJ646B12.2, 2310026M05Rik, ras homolog gene family, member U, Ryu GTPase, CDC42-like GTPase, GTP-binding protein like 1, Wnt-1 responsive Cdc42 homolog, FLJ10616, WRCH1, GTP-binding protein SB128, GTP-binding protein like 1, ras-like gene family member U
Official Symbol: RHOU
Accession Number(s): NP_067028.1
Human GeneID(s): 58480

Immunogen

Peptide with sequence PPQQGDPAFPDRCEA, from the N Terminus of the protein sequence according to NP_067028.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx. 22kDa band in lysates of cell lines A431, HeLa and HEK293 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human

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