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Goat Anti-ARPC4 Antibody (EB08249)

Related Products: EBP08249 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08249 Goat Anti-ARPC4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08249 1
EB08249 (0.03µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARPC4, actin related protein 2/3 complex, subunit 4, 20kDa, ARC20, MGC13544, p20-Arc, Arp2/3 protein complex subunit p20, actin related protein 2/3 complex subunit 4, actin related protein 2/3 complex, subunit 4 (20 kD)
Official Symbol: ARPC4
Accession Number(s): NP_005709.1
Human GeneID(s): 10093
Non-Human GeneID(s): 68089 (mouse)
Important Comments: This antibody is expected to recognise isoform a ( NP_005709.1).

Immunogen

Peptide with sequence C-ERHNKPEVEVR, from the internal region of the protein sequence according to NP_005709.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 19kDa band observed in Human Lymph Node lysates (calculated MW of 19.7kDa according to NP_005709.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

Specific Reference

This antibody has been successfully used in the following paper:
Elvira Nikalayevich, Gaëlle Letort, Ghislain de Labbey, Elena Todisco, Anastasia Shihabi, Hervé Turlier, Raphaël Voituriez, Mohamed Yahiatene, Xavier Pollet-Villard, Metello Innocenti, Melina Schuh, Marie-Emilie Terret, Marie-Hélène Verlhac
Aberrant cortex contractions impact mammalian oocyte quality.
Dev Cell. 2024 Apr 8;59(7):841-852.e7
PMID: 38387459

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