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Goat Anti-ATG4D Antibody (EB09223)

Related Products: EBP09223 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09223 Goat Anti-ATG4D Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09223 1
EB09223 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09223 2
EB09223 (2.5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09223 3
EB09223 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATG4D, ATG4 autophagy related 4 homolog D (S. cerevisiae), APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, AUT-like 4, cysteine endopeptidase, autophagin-4, autophagy-related cysteine endopeptidase 4, cysteine protease involved in autophagy
Official Symbol: ATG4D
Accession Number(s): NP_116274.3; NP_001268433.1
Human GeneID(s): 84971
Non-Human GeneID(s): 235040 (mouse), 686505 (rat)

Immunogen

Peptide with sequence CSQLAQPTLRLPRT, from the internal region (near C terminus) of the protein sequence according to NP_116274.3; NP_001268433.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 2.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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