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Goat Anti-AXIN1 Antibody (EB05380)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05380 | Goat Anti-AXIN1 Antibody | Pep-ELISA, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB05380 (3.8µg/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: AXIN1, axin 1, AXIN, LA16c-314G4.3, MGC52315, axis inhibition protein 1, axis inhibitor 1, fused, mouse, homolog ofOfficial Symbol: AXIN1
Accession Number(s): NP_003493.1; NP_851393.1
Human GeneID(s): 8312
Non-Human GeneID(s): 12005 (mouse), 79257 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms.
Immunogen
Peptide with sequence C-FEEKIIGKVEKVD, from the C Terminus of the protein sequence according to NP_003493.1; NP_851393.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6kDa according to NP_003493.1 and 91.7kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow