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Goat Anti-B56 beta isoform Antibody (EB05241)

Related Products: EBP05241 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05241 Goat Anti-B56 beta isoform Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB05241 1
EB05241 (2µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PPP2R5B, protein phosphatase 2, regulatory subunit B (B56), beta isoform, PP2A, B subunit, B\1 beta isoform, PP2A, B subunit, R5 beta isoform, PP2A, B subunit, B56 beta isoform, PP2A, B subunit, PR61 beta isoform, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, beta isoform, protein phosphatase 2, regulatory subunit B\1, beta isoform, PP2A, B subunit, R5 beta isoform, beta isoform of regulatory subunit B56, protein phosphatase 2A, protein phosphatase 2, regulatory subunit B (B56), beta isoform
Official Symbol: PPP2R5B
Accession Number(s): NP_006235.1
Human GeneID(s): 5526

Immunogen

Peptide with sequence C-QRLTPQVAASGGQS, from the C Terminus of the protein sequence according to NP_006235.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx. 55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 57.4kDa according to NP_006235.1). Recommended concentration: 2-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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