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Code Name Applications Availability Size Tested species Price Grade  
EB05486 Goat Anti-BAG3 / BIS/ CAIR1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05486 1
EB05486 (0.5µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05486 2
EB05486 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB05486 3
EB05486 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: BAG3, BCL2-associated athanogene 3, BIS, BAG-3, CAIR-1, MGC104307, DKFZp434E0610, Bcl-2-binding protein, docking protein CAIR-1, BCL2-binding athanogene 3, BAG-family molecular chaperone regulator-3
Official Symbol: BAG3
Accession Number(s): NP_004272.2
Human GeneID(s): 9531

Immunogen

Peptide with sequence C-SSMTDTPGNPAAP, from the C Terminus of the protein sequence according to NP_004272.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 80-85kDa band observed in lysates of cell line MCF7 (calculated MW of 61.6kDa according to NP_004272.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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