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Goat Anti-BAP / SIL1 Antibody (EB06071)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06071 | Goat Anti-BAP / SIL1 Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest |
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EB06071 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB06071 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06071 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: SIL1, BAP, endoplasmic reticulum chaperone SIL1, homolog of yeast, BiP-associated protein, SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae), MSS, ULG5, SIL1 proteinOfficial Symbol: SIL1
Accession Number(s): NP_071909.1; NP_001032722.1
Human GeneID(s): 64374
Important Comments: Variants (NP_071909.1; NP_001032722.1) encode the same isoform.
Immunogen
Peptide with sequence C-ELLGSVNSLLKELR, from the C Terminus of the protein sequence according to NP_071909.1; NP_001032722.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:8000.Western blot: Approx 55-60 kDa band observed in Human Liver and Kidney extracts (predicted MW of 53kDa according to NP_071909.1). Recommended concentration: 1-2µg/ml.
IHC: Paraffin embedded Human Liver and Testis. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human