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Goat Anti-BAP / SIL1 Antibody (EB06071)

Related Products: EBP06071 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06071 Goat Anti-BAP / SIL1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06071 1
EB06071 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB06071 2
EB06071 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06071 3
EB06071 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SIL1, BAP, endoplasmic reticulum chaperone SIL1, homolog of yeast, BiP-associated protein, SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae), MSS, ULG5, SIL1 protein
Official Symbol: SIL1
Accession Number(s): NP_071909.1; NP_001032722.1
Human GeneID(s): 64374
Important Comments: Variants (NP_071909.1; NP_001032722.1) encode the same isoform.

Immunogen

Peptide with sequence C-ELLGSVNSLLKELR, from the C Terminus of the protein sequence according to NP_071909.1; NP_001032722.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 55-60 kDa band observed in Human Liver and Kidney extracts (predicted MW of 53kDa according to NP_071909.1). Recommended concentration: 1-2µg/ml.
IHC: Paraffin embedded Human Liver and Testis. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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