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Goat Anti-BAT3 / BAG6 Antibody (EB09033)

Related Products: EBP09033 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09033 Goat Anti-BAT3 / BAG6 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09033 1
EB09033 (3.8µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BAT3, HLA-B associated transcript 3, BAG-6, BAG6, D6S52E, G3, HLA-B associated transcript-3, large proline-rich protein BAT3, scythe
Official Symbol: BAT3
Accession Number(s): NP_004630.3; NP_542433.1
Human GeneID(s): 7917
Non-Human GeneID(s): 224727 (mouse), 94342 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004630.3; NP_542433.1). Reported variants representing identical protein: NP_542434.1, NP_001092004.1, NP_542433.1 (isoform b).

Immunogen

Peptide with sequence C-PETNAPNHPSPAEY, from the internal region of the protein sequence according to NP_004630.3; NP_542433.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments in Human Brain (Frontal Cortex) and Human Ovary lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Spleen shows nucleoli staining in splenocytes. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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