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Goat Anti-BCAP31 / BAP31 Antibody (EB05397)

Related Products: EBP05397 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05397 Goat Anti-BCAP31 / BAP31 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05397 1
EB05397 (0.1µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05397 2
EB05397 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05397 3
EB05397 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05397 4
EB05397 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BCAP31, BAP31, DXS1357E, accessory protein BAP31, CDM, 6C6-AG, B-cell receptor-associated protein 31, CDM, 6C6 antigen, BCR-associated protein Bap31, OTTHUMP00000025978, p28 Bap31
Official Symbol: BCAP31
Accession Number(s): NP_001132929.1; NP_005736.3
Human GeneID(s): 10134
Important Comments: This antibody is expected to recognise isoform a (NP_001132929.1) and isoform b (NP_005736.3; NP_001132913.1).

Immunogen

Peptide with sequence C-QAAVDGPMDKKEE, from the C Terminus of the protein sequence according to NP_001132929.1; NP_005736.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30kDa band observed in Human Placenta and Brain lysate (predicted MW of 28kDa according to NP_005736). Recommended for use at 1-3µg/ml.
IHC: Paraffin embedded Human Kidney and Brain (Cerebellum and Cortex). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

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