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Goat Anti-BMAL1 / ARNTL Antibody (EB09178)

Related Products: EBP09178 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09178 Goat Anti-BMAL1 / ARNTL Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09178 1
EB09178 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARNTL, BMAL1, aryl hydrocarbon receptor nuclear translocator-like, BMAL1c, JAP3, MGC47515, MOP3, PASD3, TIC, ARNT-like protein 1, brain and muscle, bHLH-PAS protein JAP3, basic-helix-loop-helix-PAS orphan MOP3, member of PAS superfamily 3
Official Symbol: ARNTL
Accession Number(s): NP_001169.3; NP_001025444.1
Human GeneID(s): 406
Non-Human GeneID(s): 11865 (mouse), 29657 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001025444.1; NP_001025443.1). Reported variants represent identical protein (NP_001025443.1; NP_001169.3).

Immunogen

Peptide with sequence REKITTNCYKFKIKD, from the internal region of the protein sequence according to NP_001169.3; NP_001025444.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 70+75kDa bands observed in Human Brain (Cerebellum) lysates (calculated MW of 68.7kDa according to NP_001169.3 and 64.1kDa according to NP_001025444.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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