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Goat Anti-BMPR1A Antibody (EB07197)

Related Products: EBP07197 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07197 Goat Anti-BMPR1A Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07197 1
EB07197 (1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BMPR1A, bone morphogenetic protein receptor, type IA, HGNC:1076, ACVRLK3, ALK3, activin A receptor, type II-like kinase 3
Official Symbol: BMPR1A
Accession Number(s): NP_004320.2
Human GeneID(s): 657

Immunogen

Peptide with sequence C-KSDSDQKKSEN, from the internal region of the protein sequence according to NP_004320.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 60kDa band observed in lysates of cell lines HeLa and HepG2 (calculated MW of 60.2kDa according to NP_004320.2). Recommended concentration: 1-3µg/ml. An additional band of 20kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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