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Goat Anti-BRD4 Antibody (EB09170)

Code Name Applications Availability Size Tested species Price Grade  
EB09170 Goat Anti-BRD4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB09170 1
EB09170 (1µg/ml) staining of Mouse Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, bromodomain-containing 4, bromodomain-containing protein 4, chromosome-associated protein
Official Symbol: BRD4
Accession Number(s): NP_490597.1; NP_055114.1
Human GeneID(s): 23476
Important Comments: This antibody is expected to recognize both reported isoforms (NP_490597.1; NP_055114.1).

Immunogen

Peptide with sequence C-PETSNPNKPKRQTNQ, from the internal region (near N Terminus) of the protein sequence according to NP_490597.1; NP_055114.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 150kDa band observed in Mouse Testis lysates (calculated MW of 155.8kDa according to Mouse NP_065254.3). Recommended concentration: 0.5 -2µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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