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Goat Anti-BTBD7 Antibody (EB10630)

Related Products: EBP10630 - Immunizing Peptide, EBP10631 - Immunizing Peptide, Goat Anti-BTBD7 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10630 Goat Anti-BTBD7 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
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www.arp1.com
EB10630 1
MDCK overexpressing Mouse Btbd7 (third lane) and probed with EB10630 (mock transfection in first lane, full construct chemically suppressed in second lane).
Click here to download PDF datasheet.

Target Protein

Principal Names: BTBD7, BTB (POZ) domain containing 7, DKFZp686N0544, FUP1, MGC48310, BTB/POZ domain-containing protein 7
Official Symbol: BTBD7
Accession Number(s): NP_001002860.2; NP_060637.1
Human GeneID(s): 55727
Non-Human GeneID(s): 238386 (mouse), 362772 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001002860.2; NP_060637.1).

Immunogen

Peptide with sequence ESKLYSLDHGHEKPQ-C, from the N Terminus of the protein sequence according to NP_001002860.2; NP_060637.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: In transfected MDCK transiently expressing the fusion protein Myc-Btbd7-GFP a band of approx. 190kDa is observed. This band is also observed by Myc-antibody and it is not observed in the MDCK expressing GFP, or when the fusion gene was suppressed. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY. This antibody has been successfully used in WB on Mouse: PMID: 31303496. Grisard E et al. EBioMedicine. 2019 Aug;46:79-93.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Grisard E, Coan M, Cesaratto L, Rigo I, Zandonà L, Paulitti A, Andreuzzi E, Rampioni Vinciguerra GL, Poletto E, Del Ben F, Brisotto G, Biscontin E, Turetta M, Dassi E, Mirnezami A, Canzonieri V, Vecchione A, Baldassarre G, Mongiat M, Spizzo R, Nicoloso MS
Sleeping beauty genetic screen identifies miR-23b::BTBD7 gene interaction as crucial for colorectal cancer metastasis.
EBioMedicine. 2019 Aug;46:79-93
PMID: 31303496

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