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Goat Anti-CAPON / NOS1AP (internal) Antibody (EB07119)

Related Products: EBP05583 - Immunizing Peptide, EBP07119 - Immunizing Peptide, Goat Anti-CAPON / NOS1AP Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07119 Goat Anti-CAPON / NOS1AP (internal) Antibody Pep-ELISA Allow up to 10 days for delivery 100µg specific antibody in 200µl $248.00 Aspiring

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: NOS1AP, nitric oxide synthase 1 (neuronal) adaptor protein, CAPON, 6330408P19Rik, C-terminal PDZ domain ligand of neuronal nitric oxide synthase, C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON), ligand of neuronal nitric oxide synthase with carboxyl-terminal PDZ domain
Official Symbol: NOS1AP
Accession Number(s): NP_055512.1
Human GeneID(s): 9722
Non-Human GeneID(s): 70729 (mouse), 192363 (rat)

Immunogen

Peptide with sequence CNVFKSKKKSQ, from the internal region of the protein sequence according to NP_055512.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Western Blot: Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Brain (Frontal Cortex) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 56.1kDa according to NP_055512.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog

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