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Goat Anti-CASP4 Antibody (EB06481)

Related Products: EBP06481 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06481 Goat Anti-CASP4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06481 1
EB06481 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CASP4, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, caspase 4, apoptosis-related cysteine protease, apoptotic cysteine protease Mih1/TX, caspase 4
Official Symbol: CASP4
Accession Number(s): NP_001216.1; NP_150649.1
Human GeneID(s): 837
Important Comments: This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1).

Immunogen

Peptide with sequence CKERAEEIYPIKERN, from the internal region of the protein sequence according to NP_001216.1; NP_150649.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40kDa band observed in Human Heart lysates (calculated MW of 43.3kDa according to NP_001216.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Cat, Dog, Cow

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