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Goat Anti-Catenin alpha1 Antibody (EB07242)

Related Products: EBP07242 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07242 Goat Anti-Catenin alpha1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07242 1
EB07242 (1µg/ml) staining of human colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07242 2
HEK293 overexpressing Human CTNNA1 (RC201766) and probed with EB07242 (mock transfection in first lane), tested by Origene.
EB07242 3
EB07242 (3.75µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07242 4
EB07242 (3.75µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: catenin (cadherin-associated protein), alpha 1, 102kDa, HGNC:2509, CTNNA1, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-catenin, alpha-catenin, alphaE-catenin, cadherin-associated protein,102kDa, catenin (cadherin-associated protein), alpha 1 (102kD), catenin, alpha 1
Official Symbol: CTNNA1
Accession Number(s): NP_001894.2
Human GeneID(s): 1495
Non-Human GeneID(s): 12385 (mouse), 307505 (rat)

Immunogen

Peptide with sequence C-KREKQDETQTKIK, from the internal region of the protein sequence according to NP_001894.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100kDa band observed in human colon and human skin lysates (calculated MW of 100kDa according to NP_001894.2). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Prostate and Uterus. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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