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Goat Anti-CD56 / NCAM1 (isoform 2) Antibody (EB10948)

Related Products: EBP10948 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10948 Goat Anti-CD56 / NCAM1 (isoform 2) Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10948 1
EB10948 (0.01µg/ml) staining of Human Frontal Cortex (A) and (0.1ug/ml) Cerebellum (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10948 2
EB10948 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB10948 3
EB10948 Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: NCAM1, neural cell adhesion molecule 1, CD56, MSK39, NCAM, N-CAM-1, NCAM-1, antigen recognized by monoclonal antibody 5.1H11, neural cell adhesion molecule, NCAM
Official Symbol: NCAM1
Accession Number(s): NP_851996.2
Human GeneID(s): 4684
Non-Human GeneID(s): 24586 (rat)
Important Comments: This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.

Immunogen

Peptide with sequence C-KASWTRPEKQETLD, from the internal region of the protein sequence according to NP_851996.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150-170kDa band observed in Human Cerebellum and Frontal Cortex lysates (calculated MW of 94.6kDa according to NP_851996.2). The observed molecular weight corresponds to the glycosylated form and is routinely observed by other sources. Recommended concentration: 0.03-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Pig, Cow

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