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Goat Anti-CD80 Antibody (EB09701)

Related Products: EBP09701 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09701 Goat Anti-CD80 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09701 1
EB09701 (0.5µg/ml) staining of K562 (A) and U937 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09701 2
EB09701 (3ug/ml) staining of Human Lymph Node (A), Bone Marrow (B) and (1.5ug/ml) Spleen (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09701 3
EB09701 (6µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09701 4
EB09701 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
EB09701 5
EB09701 (5µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09701 6
EB09701 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
EB09701 7
EB09701 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD80, CD80 molecule, CD28LG, CD28LG1, LAB7, B-lymphocyte activation antigen B7, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), costimulatory factor CD80, costimulatory molecule variant IgV-CD80
Official Symbol: CD80
Accession Number(s): NP_005182.1
Human GeneID(s): 941

Immunogen

Peptide with sequence C-HLRVNQTFNWNTTK, from the internal region of the protein sequence according to NP_005182.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx. 65kDa band observed in lysates of cell lines K562 and U937, and approx. 60kDa in Human Lymph Node, Bone Marrow and Spleen lysates (calculated MW of 33.0kDa according to NP_005182.1). These molecular weights are routinely observed by other sources. Recommended concentration: 0.5-3µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.


IHC: Paraffin embedded Human Spleen and Tonsil. Recommended concentration: 4-5µg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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