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Goat Anti-CDYL Antibody (EB05779)

Related Products: EBP05779 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05779 Goat Anti-CDYL Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05779 1
EB05779 staining (1µg/ml) of Human Testes lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CDYL, chromodomain protein, Y-like, CDYL1, DKFZP586C1622, MGC131936, CDY-like, autosomal, bA620A17.2 (chromodomain protein, Y chromosome-like), chromodomain protein, Y chromosome-like, chromodomain protein, Y chromosome-like, isoform b, testis-specific chromodomain Y-like protein, DKFZp586C1622
Official Symbol: CDYL
Accession Number(s): NP_004815.3; NP_001137442.1; NP_001137443.1
Human GeneID(s): 9425
Non-Human GeneID(s): 12593 (mouse)
Important Comments: This antibody is expected to recognize both eported isoforms NP_004815.3; NP_001137442.1; NP_001137443.1). Variants (NP_001137442.1; NP_001137443.1) encode the same isoform.

Immunogen

Peptide with sequence C-SMLKYLQRKIDEF, from the C Terminus of the protein sequence according to NP_004815.3; NP_001137442.1; NP_001137443.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8707-12; PMID: 12072557). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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