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Goat Anti-CHRNB1 / ACHRB Antibody (EB08494)

Related Products: EBP08494 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08494 Goat Anti-CHRNB1 / ACHRB Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08494 1
EB08494 (1µg/ml) staining of human cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CHRNB1, ACHRB, cholinergic receptor, nicotinic, beta 1 (muscle), CHRNB, CMS1D, CMS2A, SCCMS, cholinergic receptor, nicotinic, beta polypeptide 1 (muscle), nicotinic acetylcholine receptor beta 1 subunit
Official Symbol: CHRNB1
Accession Number(s): NP_000738.2
Human GeneID(s): 1140

Immunogen

Peptide with sequence C-QEQEDHDALKED, from the internal region of the protein sequence according to NP_000738.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 55kDa band observed in human brain (cerebellum, hippocampus, frontal cortex) lysates (calculated MW of 56.7kDa according to NP_000738.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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