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Goat Anti-CHRNB2 Antibody (EB08480)

Related Products: EBP08480 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08480 Goat Anti-CHRNB2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08480 1
EB08480 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08480 2
EB08480 (2.5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CHRNB2, cholinergic receptor, nicotinic, beta 2 (neuronal), EFNL3, nAChRB2, cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal), neuronal nicotinic acetylcholine receptor beta 2
Official Symbol: CHRNB2
Accession Number(s): NP_000739.1
Human GeneID(s): 1141

Immunogen

Peptide with sequence QPRHHCARQRLR, from the internal region of the protein sequence according to NP_000739.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 40-48 kDa band observed in Human Brain (Cerebellum),) lysates (calculated MW of 57.0 kDa according to NP_000739.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Uterus. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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