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Code Name Applications Availability Size Tested species Price Grade  
EB05738 Goat Anti-CIAS1 / cryopyrin ( C Terminus) Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05738 1
EB05738 Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05738 2
EB05738 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05738 3
EB05738 (4µg/ml) staining of paraffin embedded Human Colon. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05738 4
EB05738 Negative Control showing staining of paraffin embedded Human Colon, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: NLRP3, CIAS1, FCU, MWS, FCAS, NALP3, C1orf7, PYPAF1, AII/AVP, AGTAVPRL, CRYOPYRIN, cold autoinflammatory syndrome 1, cryopyrin, Muckle-Wells syndrome, chromosome 1 open reading frame 7, PYRIN-containing APAF1-like protein 1, neuronal apoptosis inhibitor protein 3, angiotensin/vasopressin receptor AII/AVP-like, NLR family, pyrin domain containing 3, AII, AVP, CLR1.1, FLJ95925, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Official Symbol: NLRP3
Accession Number(s): NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1
Human GeneID(s): 114548
Important Comments: Variants (NP_001073289.1; NP_004886.3) encode the same isoform.

Immunogen

Peptide with sequence C-EKPELTVVFEPSW, from the C Terminus of the protein sequence according to NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: This antibody is not recommended for use in Western blotting.
IHC: Paraffin embedded Human Colon. Recommended concentration: 4µg/ml.

Immunofluorescence: Expression of the protein seen in the cytoplasm and plasma membrane of U937 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Cow

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