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Code Name Applications Availability Size Tested species Price Grade  
EB05738 Goat Anti-CIAS1 / cryopyrin ( C Terminus) Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05738 1
EB05738 Flow cytometric analysis of paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. NB: Gated on Granulocytes.
EB05738 2
EB05738 (5µg/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: NLRP3, CIAS1, FCU, MWS, FCAS, NALP3, C1orf7, PYPAF1, AII/AVP, AGTAVPRL, CRYOPYRIN, cold autoinflammatory syndrome 1, cryopyrin, Muckle-Wells syndrome, chromosome 1 open reading frame 7, PYRIN-containing APAF1-like protein 1, neuronal apoptosis inhibitor protein 3, angiotensin/vasopressin receptor AII/AVP-like, NLR family, pyrin domain containing 3, AII, AVP, CLR1.1, FLJ95925, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Official Symbol: NLRP3
Accession Number(s): NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1
Human GeneID(s): 114548
Important Comments: Variants (NP_001073289.1; NP_004886.3) encode the same isoform.

Immunogen

Peptide with sequence C-EKPELTVVFEPSW, from the C Terminus of the protein sequence according to NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: This antibody is not recommended for use in Western blotting.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of human peripheral blood monocyte cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Cow

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