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Goat Anti-CLIC4 Antibody (EB07738)

Related Products: EBP07738 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07738 Goat Anti-CLIC4 Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07738 1
EB07738 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07738 2
EB07738 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07738 3
EB07738 (5ug/ml) staining of min6 cells transiently expressing mouse Clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.
Click here to download PDF datasheet.

Target Protein

Principal Names: CLIC4, chloride intracellular channel 4, CLIC4L, DKFZP566G223, FLJ38640, H1, huH1, p64H1, chloride intracellular channel 4 like
Official Symbol: CLIC4
Accession Number(s): NP_039234.1
Human GeneID(s): 25932
Non-Human GeneID(s): 29876 (mouse), 83718 (rat)

Immunogen

Peptide with sequence NGLKEEDKEPLIE-C, from the N Terminus of the protein sequence according to NP_039234.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 30kDa band observed in Human Kidney and Placenta lysates (calculated MW of 28.8kDa according to NP_039234.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J Physiol. 1999 Mar;276(3 Pt 2):F398-408.; PMID: 10070163). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration, 5µg/ml with overnight incubation.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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