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Code Name Applications Availability Size Tested species Price Grade  
EB07357 Goat Anti-COMT (internal) Antibody Pep-ELISA, WB, EIA, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
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EB07357 1
EB07357 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07357 2
EB7357 (0.5ug/ml) as the reporter with EB002002 as the capture rabbit antibody (2.5ug/ml).
EB07357 3
EB07357 (4µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
EB07357 4
EB07357 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07357 5
EB07357 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: COMT, catechol-O-methyltransferase
Official Symbol: COMT
Accession Number(s): NP_000745.1; NP_009294.1
Human GeneID(s): 1312
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)

Immunogen

Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1; NP_009294.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 28kDa band in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1µg/ml.
IHC: In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Testis and Small Intestine. Recommended concentration: 3.75µg/ml.

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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