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|EB07553||Goat Anti-CRHR1 / CRF-R (aa 250 - 263) Antibody||Pep-ELISA, WB, IF, IHC||100µg specific antibody in 200µl||Human||$315.00|
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Peptide with sequence KLYYDNEKCWFGKR, from the internal region of the protein sequence according to NP_004373.2.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence: Expression of the protein seen in the cytoplasm and endosomes of MCF7 cells. Recommended concentration: 10µg/ml.
This antibody has been successfully used in Western blot on Mouse:
Hunt AJ Jr, Dasgupta R, Rajamanickam S, Jiang Z, Beierlein M, Chan CS, Justice NJ
Paraventricular hypothalamic and amygdalar CRF neurons synapse in the external globuspallidus.
Brain Struct Funct. 2018 Jul;223(6):2685-2698.
This antibody (previous batch) has been successfully used in IF on Mouse:
Jiang Z, Rajamanickam S, Justice NJ.
Local Corticotropin-Releasing Factor Signaling in the Hypothalamic Paraventricular Nucleus.
J Neurosci. 2018 Feb 21;38(8):1874-1890.
This antibody (previous batch) has been successfully used in ICC on Rat:
Adrienne L. Andres, Limor Regev, Lucas Phi, Ronald R. Seese, Yuncai Chen, Christine M. Gall and Tallie Z. Baram
NMDA Receptor Activation and Calpain Contribute to Disruption of Dendritic Spines by the Stress Neuropeptide CRH
J Neurosci. 2013 Oct 23;33(43):16945-60.