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Code Name Applications Availability Size Tested species Price Grade  
EB05072 Goat Anti-DAPP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05072 1
EB05072 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DAPP1, dual adaptor of phosphotyrosine and 3-phosphoinositides, BAM32, DKFZp667E0716, HSPC066, B-cell adapter molecule of 32 kDa, b lymphocyte adapter protein Bam32, dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1
Official Symbol: DAPP1
Accession Number(s): AAF14578.1
Human GeneID(s): 27071
Important Comments: This antibody was raised against the C-terminus of NP_055210.1. However, NCBI has changed the version to NP_055210.2 thus losing the C-terminal epitope.

Immunogen

Peptide with sequence SALCISPEEKTDHK, from the C Terminus of the protein sequence according to AAF14578.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 30kDa band observed in Human Kidney lysates (calculated MW of 32.0kDa according to AAF14578.1). Recommended concentration: 0.5-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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