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Goat Anti-DCTN3 Antibody (EB10446)

Related Products: EBP10445 - Immunizing Peptide, EBP10446 - Immunizing Peptide, Goat Anti-DCTN3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10446 Goat Anti-DCTN3 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Rat $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10446 1
EB10446 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescenceWS10446-4
EB10446 2
EB10446 (0.3µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
EB10446 3
EB10446 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB10446 4
EB10446 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
EB10446 5
EB10446 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DCTN22, DCTN-22, dynactin 3, dynactin 3 (p22), dynactin 3, isoform 1, dynactin light chain, MGC111190, DCTN3
Official Symbol: DCTN3
Accession Number(s): NP_009165.1; NP_077324.1
Human GeneID(s): 11258
Non-Human GeneID(s): 53598 (mouse), 362504 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).

Immunogen

Peptide with sequence C-QVALGNISSKRE, from the internal region of the protein sequence according to NP_009165.1; NP_077324.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 20kDa band observed in Human Liver lysates, and approx. 18kDa in Rat Kidney lysates and in preliminary testing of Mouse Kidney lysate (calculated MW of 19.5kDa according to Human NP_077324.1 and 21.1kDa according to Rat NP_001102129.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 6-8µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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