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Goat Anti-Dipeptidylpeptidase 10 Antibody (EB06750)

Related Products: EBP06750 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06750 Goat Anti-Dipeptidylpeptidase 10 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06750 1
EB06750 (0.1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06750 2
EB06750 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06750 3
EB06750 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DPP10, dipeptidylpeptidase 10, HGNC:20823, DPRP3, dipeptidyl peptidase IV-related protein 3, dipeptidyl peptidase X, dipeptidyl peptidase-like protein 2, dipeptidyl-peptidase 10, DPL2, DPPY, dipeptidyl peptidase 10, dipeptidyl peptidase like protein 2
Official Symbol: DPP10
Accession Number(s): NP_065919.2; NP_001004360.2
Human GeneID(s): 57628
Important Comments: This antibody is expected to recognise both reported isoforms NP_065919.2 and NP_001004360.2.

Immunogen

Peptide with sequence C-DEGHNVSEKSKYHL, from the internal region of the protein sequence according to NP_065919.2; NP_001004360.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human Bone Marrow lysates (calculated MW of 90.0kDa according to NP_065919.2 and of 90.1 according to NP_001004360.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Brain (Cortex and Cerebellum). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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