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Goat Anti-DKC1 Antibody (EB07703)

Code Name Applications Availability Size Tested species Price Grade  
EB07703 Goat Anti-DKC1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB07703 1
EB07703 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKC1, dyskeratosis congenita 1, dyskerin, DKC, NAP57, NOLA4, XAP101
Official Symbol: DKC1
Accession Number(s): NP_001354.1
Human GeneID(s): 1736
Non-Human GeneID(s): 245474 (mouse)

Immunogen

Peptide with sequence C-KRKRESESESDETPP, from the internal region of the protein sequence according to NP_001354.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 70-75kDa band in Human Bone Marrow, Duodenum and Skin lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7kDa according to NP_001354.1. The 70-75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Spleen shows strong nuclear staining of select splenocytes. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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