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Goat Anti-Dnajc5 (aa177-191) Antibody (EB11846)

Related Products: EBP11845 - Immunizing Peptide, EBP11846 - Immunizing Peptide, Goat Anti-Dnajc5 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11846 Goat Anti-Dnajc5 (aa177-191) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11846 1
EB11846 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11846 2
EB11846 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: 2610314I24Rik, AU018536, Csp, cysteine string protein, DnaJ (Hsp40) homolog, subfamily C, member 5, dnaJ homolog subfamily C member 5, Dnajc5
Official Symbol: Dnajc5
Accession Number(s): NP_058055.1
Human GeneID(s): 80331
Non-Human GeneID(s): 13002 (mouse), 79130 (rat)

Immunogen

Peptide with sequence C-SATETTQLTADSHPS, from the C Terminus of the protein sequence according to NP_058055.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 20kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1kDa according to NP_058055.1). Recommended concentration: 0.5-1.5µg/ml. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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