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Goat Anti-Endothelial lipase Antibody (EB08961)

Related Products: EBP08961 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08961 Goat Anti-Endothelial lipase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08961 1
EB08961 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: endothelial lipase, lipase, endothelial, LIPG, EDL, EL, PRO719, endothelial cell-derived lipase, lipoprotein lipase H
Official Symbol: LIPG
Accession Number(s): NP_006024.1
Human GeneID(s): 9388

Immunogen

Peptide with sequence C-RFNLRTSKDPEHEG, from the internal region of the protein sequence according to NP_006024.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 70kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_006024.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gauster et al , J Clin Endocrinol Metab. 2007 Jun;92(6):2256-63. Epub 2007 Mar 13.; PMID: 17356047). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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