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Goat Anti-ERK2 / MAPK1 Antibody (EB06592)

Related Products: EBP06592 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06592 Goat Anti-ERK2 / MAPK1 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06592 1
EB06592 (0.5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06592 2
EB06592 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: MAPK1, ERK2, ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2, mitogen-activated protein kinase 2, extracellular signal-regulated kinase 2
Official Symbol: MAPK1
Accession Number(s): NP_002736.3; NP_620407.1
Human GeneID(s): 5594
Non-Human GeneID(s): 26413 (mouse)
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.

Immunogen

Peptide with sequence CAAGPEMVRGQVF, from the internal region of the protein sequence according to NP_002736.3; NP_620407.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 38kDa band observed in HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2µg/ml. Primary incubation was 1 hour.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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