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Goat Anti-ETFA (aa139-152) Antibody (EB10173)

Related Products: EBP10173 - Immunizing Peptide, EBP10174 - Immunizing Peptide, Goat Anti-ETFA Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10173 Goat Anti-ETFA (aa139-152) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10173 1
EB10173 (0.1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10173 2
EB10173 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB10173 3
EB10173 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: electron transfer flavoprotein alpha-subunit, electron transfer flavoprotein, alpha polypeptide, electron-transfer-flavoprotein, alpha polypeptide, EMA, GA2, glutaric aciduria II, MADD, ETFA
Official Symbol: ETFA
Accession Number(s): NP_000117.1; NP_001121188.1
Human GeneID(s): 2108
Non-Human GeneID(s): 110842 (mouse), 300726 (rat)
Important Comments: This antibody is expected to recognize both isoforms (NP_000117.1; NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)

Immunogen

Peptide with sequence C-KSPDTFVRTIYAGN, from the internal region of the protein sequence according to NP_000117.1; NP_001121188.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 30+35kDa bands observed in Human Colon lysates (calculated MW of 35.1kDa according to NP_000117.1 and of 30.0kDa according to NP_001121188.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Adrenal Gland and Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Tomilov A, Tomilova N, Shan Y, Hagopian K, Bettaieb A, Kim K, Pelicci PG, Haj F, Ramsey J, Cortopassi G.
The mitochondrial isoform of Shc protein inhibits thiolase and lipid oxidation.
J Biol Chem. 2016 Apr 8.
PMID: 27059956

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