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Code Name Applications Availability Size Tested species Price Grade  
EB07434 Goat Anti-FLAP / ALOX5AP (internal) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07434 1
EB07434 (0.1µg/ml) staining of Human U937 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07434 2
EB07434 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07434 3
EB07434 (3.75µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07434 4
EB07434 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ALOX5AP, FLAP, arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein, five-lipoxygenase activating protein
Official Symbol: ALOX5AP
Accession Number(s): NP_001620.2; NP_001191335.1
Human GeneID(s): 241
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001620.2; NP_001191335.1).

Immunogen

Peptide with sequence C-HKVEHESRTQNGR, from the internal region of the protein sequence according to NP_001620.2; NP_001191335.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 16kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2kDa according to NP_001620.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Spleen shows staining of select splenocytes. Paraffin embedded Human Lung and Small Intestine. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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