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Code Name Applications Availability Size Tested species Price Grade  
EB06000-B Goat Anti-FOXA2 / HNF3B, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06000-B 1
Biotinylated EB06000 (1.5µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXA2, forkhead box A2, HNF3B, TCF3B, HNF-3-beta, HNF-3B, TCF-3B, forkhead box protein A2, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta, transcription factor 3B, MGC19807
Official Symbol: FOXA2
Accession Number(s): NP_068556.2; NP_710141.1;
Human GeneID(s): 3170
Non-Human GeneID(s): 15376 (mouse), 25099 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms: NP_068556.2; NP_710141.1.

Immunogen

Peptide with sequence C-GVYSRPIMNSS., from the C Terminus of the protein sequence according to NP_068556.2; NP_710141.1;.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 60kDa band observed in Rat Lung lysates (calculated MW of 48.3kDa according to Human NP_710141.1 and 48.5kDa according to Rat NP_036875.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277(20):17564-70.; PMID: 11875061). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human

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