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Goat Anti-FOXP3 / Scurfin (Mouse) Antibody (EB06376)

Related Products: EBP06376 - Immunizing Peptide, Goat Anti-FOXP3 / Scurfin (Mouse) Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06376 Goat Anti-FOXP3 / Scurfin (Mouse) Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Mouse $450.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06376 1
EB06376 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06376 2
EB06376 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06376 3
EB06376 (5µg/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06376 4
EB06376 Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: Foxp3, forkhead box P3, sf, JM2, scurfin, scurfy, RP23-54C14.1, OTTMUSP00000017952, OTTMUSP00000017961, OTTMUSP00000017962
Official Symbol: Foxp3
Accession Number(s): NP_473380.1
Non-Human GeneID(s): 20371 (mouse), 317382 (rat)
Important Comments: Note - anti-mouse companion product of EB05294

Immunogen

Peptide with sequence KRSQRPNKCSNP, from the C Terminus of the protein sequence according to NP_473380.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Mouse Spleen. Recommended concentration: 5-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei of NIH3T3 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Mouse, Rat

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