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Goat Anti-GCC185 Antibody (EB07992)

Related Products: EBP07992 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07992 Goat Anti-GCC185 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07992 1
EB07992 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07992 2
EB07992 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07992 3
EB07992 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GCC2, GRIP and coiled-coil domain containing 2, GCC185, KIAA0336, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GRIP and coiled-coil domain-containing 2, GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4)
Official Symbol: GCC2
Accession Number(s): NP_055450.1; NP_852118.1
Human GeneID(s): 9648
Important Comments: This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).

Immunogen

Peptide with sequence C-QKLTNKNNKIED, from the internal region of the protein sequence according to NP_055450.1; NP_852118.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 60kDa band in Human Lymph Node and Testis lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kDa according to NP_055450.1 and of 196kDa according to NP_852118.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Spleen, Small Intestine and Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat

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