Change country
Home

Goat Anti-GDF15 Antibody (EB06909)

Code Name Applications Availability Size Tested species Price Grade  
EB06909 Goat Anti-GDF15 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $324.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06909 1
EB06909 (0.3µg/ml) staining of Human Testes (A) and negative control Skin (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06909 2
HEK293 overexpressing GDF15 (RC201295) and probed with EB06909 (mock transfection in first lane), tested by Origene.
EB06909 3
EB06909 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06909 4
EB06909 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06909 5
EB06909 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, growth differentiation factor 15, PTGF-beta, prostate differentiation factor, NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1
Official Symbol: GDF15
Accession Number(s): NP_004855.2
Human GeneID(s): 9518

Immunogen

Peptide with sequence C-QKTDTGVSLQTYDD, from the C Terminus of the protein sequence according to NP_004855.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 30-35 kDa band observed in Human Testis lysates (calculated MW of 34.1kDa according to NP_004855.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Skin lysate. In transfected HEK293 transiently expressing GDF15 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293. Preliminary testing was unsuccessful on Mouse for this particular batch.
IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Product Reviews

Please login to review this product.