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Goat Anti-GRAP2 / GRID / Grf40 Antibody (EB05113)

Related Products: EBP05113 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05113 Goat Anti-GRAP2 / GRID / Grf40 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05113 1
HEK293 overexpressing GRAP2 (RC206546) and probed with EB05113 (mock transfection in first lane), tested by Origene.
EB05113 2
EB05113 (0.3µg/ml) staining of Jurkat lysate (30µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRAP2, GRID, P38, GADS, GRPL, GrbX, Mona, GRB2L, GRBLG, GRAP-2, GRB2-related adaptor protein 2, GRB-2-like protein, SH3-SH2-SH3 adaptor molecule, growth factor receptor-binding protein, GRB2-related protein with insert domain, hematopoietic cell-associated adaptor protein GRPL, growth factor receptor-bound protein 2-related adaptor protein 2, RP3-370M22.1, Grf40, Mona
Official Symbol: GRAP2
Accession Number(s): NP_004801.1
Human GeneID(s): 9402
Non-Human GeneID(s): 17444 (mouse)

Immunogen

Peptide with sequence C-GLFPANYVAPMTR, from the C Terminus of the protein sequence according to NP_004801.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38kDa band observed lysates of cell line Jurkat (calculated MW of 37.9kDa according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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