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Goat Anti-GRB7 (N Terminus) Antibody (EB05417)

Code Name Applications Availability Size Tested species Price Grade  
EB05417 Goat Anti-GRB7 (N Terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB05417 1
EB05417 (1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRB7, growth factor receptor-bound protein 7, OTTHUMP00000164352
Official Symbol: GRB7
Accession Number(s): NP_005301.2
Human GeneID(s): 2886
Non-Human GeneID(s): 14786 (mouse)
Important Comments: This antibody recognises the longer isoform of this protein in A431 lysate.

Immunogen

Peptide with sequence ELDLSPPHLSSSPE-C, from the N Terminus of the protein sequence according to NP_005301.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 65kDa band observed in lysates of cell line A431 (calculated MW of 59.7kDa according to NP_005301.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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