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Goat Anti-HNF4A Antibody (EB06700)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06700 | Goat Anti-HNF4A Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB06700 (0.1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06700 (2µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12Official Symbol: HNF4A
Accession Number(s): NP_849180.1; NP_000448.3; NP_849181.1
Human GeneID(s): 3172
Important Comments: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
Immunogen
Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1; NP_000448.3; NP_849181.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 55kDa and 48kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 2µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Dog, Pig