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Goat Anti-HNF4A Antibody (EB06700)

Related Products: EBP06700 - Immunizing Peptide, EBP07649 - Immunizing Peptide, Goat Anti-HNF4A Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06700 Goat Anti-HNF4A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06700 1
EB06700 (0.1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06700 2
EB06700 (2µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12
Official Symbol: HNF4A
Accession Number(s): NP_849180.1; NP_000448.3; NP_849181.1
Human GeneID(s): 3172
Important Comments: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).

Immunogen

Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1; NP_000448.3; NP_849181.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa and 48kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig

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